murine MAb against the 41-kDa flagellin of Borrelia burgdorferi, Clone LA-10

Description
Lyme disease is the most common vector-borne disease in North America and Europe. The causative agent Borrelia burgdorferi is a bacterium that is maintained in an enzoonotic cycle between Ixodes ticks and a large range of mammals. The 41 kDa flagellin of Borrelia burgdorferi is a major component of periplasmic flagellar filament core and a good candidate for serodiagnosis in early stage of Lyme disease.

Properties
The monoclonal antibody ADG92L (clone LA-10) is a murine monoclonal antibody, subclass IgG_2a recognizing flagellin. Mice were immunized with cell lysates of Borrelia burgdorferi. The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.

Presentation
Vial containing 1 mg purified antibody in PBS pH 7.4. The concentration is given on the vial label. Spin the vial briefly before opening.

Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at –20°C or colder. It is recommended to avoid freeze-thaw cycles.

Applications
ELISA
The antibody can be used as capture antibody in ELISAs. An antibody concentration of 1-10 µg/ml is recommended.
Westernblot
The antibody is suitable for Western blot analysis, detecting native and recombinant lpLA7 following SDS-PAGE under reducing conditions. A primary antibody concentration of 1-10 µg/mL is recommended.
Immunocytochemistry
The antibody can be used for immunocytochemistry on paraformaldehyde fixed.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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