OLIGOBIND® Thrombin activity assay

INTENDED USE
The OLIGOBIND^® Thrombin activity assay is an enzyme-capture-assay for the quantitative measurement of active thrombin in stabilized plasma samples.  

EXPLANATION OF THE TEST
The conversion of prothrombin to thrombin is a key event in thrombus (clot) formation. Thrombin is a serine protease that acts on a wide variety of substrates in the coagulation pathway. In vivo generated thrombin can be indirectly assessed by the measurement of prothrombin fragment F1.2, an activation peptide generated during the conversion of prothrombin to thrombin, or thrombin-antithrombin-complexes (TAT), formed during the inactivation of thrombin by its major plasma inhibitor antithrombin. However, due to differential accumulation in the circulation, these parameters do not reflect the current state level of functional active thrombin in vivo. In combination with the Thrombin blood collection tubes (Product No. ADG844T) that ensure the stabilization of thrombin-activity ex vivo, the OLIGOBIND^® Thrombin activity assay allows the direct quantification of functional active thrombin in plasma from peripheral blood.         

PRINCIPLE OF THE METHOD
Stabilized plasma samples are added to microwells coated with a DNA-aptamer against thrombin. During an incubation period, thrombin present in the sample will bind to the aptamer coated to the wells. Following a washing step, a fluorogenic peptide substrate for thrombin is added to the micro-wells. Measuring the change of fluorescence (360_[ex]/460_[em] nm) and extra-polating the value with those of a standard curve determines the level of thrombin in the plasma sample.

Category: Research use only

Type: ELISA

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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