murine MAb against uPA B-chain, affinity purified
Description
The monoclonal antibody ADG3689 (clone HD-UK1, subclass IgG1) is a murine monoclonal antibody recognizing human urokinase type plasminogen activator (uPA). The antibody has been purified from cell culture supernatant using Protein G affinity chromatography.
Properties
ADG3689 is directed against a B-chain epitope of human urokinase, near the catalytic site. The antibody reacts with free, and receptor bound, single and two chain (HMW) urokinase, uPA/PAI-1 and, uPA/PAI-2 complexes and the B-chain (33 kDa) fragment.
Presentation
Screw capped vial containing 250 µg of purified antibody in PBS pH 7.4, 0.01 % ProClin300. The IgG concentration is 1 mg/ml. Spin the vial briefly before opening.
Storage and Stability
Store the antibody at 2°-8°C. For long-term storage the antibody should be aliquoted and stored at
-20°C or colder. It is recommended to avoid freeze-thaw cycles.
Applications
A. ELISA
The antibody can be used as detecting antibody in ELISAs. An antibody concentration of 1µg/ml is recommended (1, 2).
B. Immunohistochemistry
The antibody is suitable for staining frozen acetone fixed skin sections (3), and formalin fixed, paraffin embedded sections of cancerous and normal human breast and colon tissue where urokinase is present. Working dilutions range from 1:100 (4) to 1:25 (6).
C. Flow cytometry
The antibody can be used for flow cytometric analysis. An antibody concentration of 10 µg/ml is recommended (7).
D. Western Blot Analysis
The antibody is suitable for Western blot analysis, detecting pro-uPA, HMW-uPA and LMW-uPA after electrophoresis under non-reduced conditions and B-chain fragments under reducing conditions at a working concentration of 1 μg/mL (8).
E. Inhibition
The antibody was shown to inhibit plasminogen activation on the surface of melanoma cell lines (9).
Category: Research use only
Type: Antibody
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
murine MAb against uPA B-chain, affinity purified, clone HD-UK1
Cat.No. ADG3689
Article no.: 938886
Unit: 250 µg
Code: ADG3689
Manufacturer: ImmBioMed GmbH & Co. KG
References
- Plasminogen activation in lesional skin of Pemphigus vulgaris type Neumann. Reinartz J et al., Arch Dermatol Res. 1993;284(8):432-439.
- Enzyme-linked immunosorbent assays for plasminogen activators. Buessecker F et al., J Immunol Methods. 1993 Jun 18;162(2):193-200.
- Plasminogen activation in bullous pemphigoid immunohistology reveals urokinase type plasminogen activator, its receptor and plasminogen activator inhibitor type-2 in lesional epidermis. Schaefer BM et al., Autoimmunity. 1996;23(3):155-164.
- Combined overexpression of urokinase, urokinase receptor, and plasminogen activator inhibitor-1 is associated with breast cancer progression: an immunohistochemical comparison of normal, benign, and malignant breast tissues. Costantini V et al., Cancer. 1996 Mar 15; 77(6):1079-1088.
- Immunolocalization of urokinase-type plasminogen activator in adenomas and carcinomas of the colorectum. Sier CF et al., Histopathology. 1991 Sep;19(3):231-237.
- Expression and localization of elements of the plasminogen activation system in benign breast disease and breast cancers. Jankun J et al., J Cell Biochem. 1993 Oct;53(2):135-144.
- Monoclonal antibodies against plasminogen activators and plasmin(ogen). Kramer MD et al, Fibrinolysis. 1991 6 Suppl. 4: 103-111.
- Cathepsin B efficiently activates the soluble and the tumor cell receptor-bound form of the proenzyme urokinase-type plasminogen activator (Pro-uPA). Kobayashi H et al., J Biol Chem. 1991 Mar 15;266(8):5147-5152.
- Generation of cell surface-bound plasmin by cell-associated urokinase-type or secreted tissue-type plasminogen activator: a key event in melanoma cell invasiveness in vitro. Meissauer A et al., Exp Cell Res. 1992 Apr;199(2):179-190.