RVV-X (Russell's Viper Venom, factor X activating protein)
Description
Specific Factor X activator from Russell’s viper venom (RVV-X). RVV-X is a dimer of two peptide chains with a molecular weight of 60 kDa each. Activation of factor X by the activator strictly depends on the presence of calcium ions.
Applications
RVV-X is used in diagnostic procedures to quantitatively convert the zymogen factor X into factor Xa which can be determined by means of a clotting assay or photometrically, using a synthetic chromogenic substrate. RVV-X activator is used in testing of lupus anti-coagulants.
Assays
Chromogenic method
100 µl RVV-X (25 U/ml 25 mM CaCl2)
10 µl human citrated plasma
=> incubate for 75 sec at 37°C to activate factor X
into factor Xa
790 µl 50 mM Tris-HCl, pH 8.4
100 µl SPECTROZYME® FXa substrate (4 mM)
=> Determination of ΔOD/min at 405 nm
Clotting method
100 µl RVV-X (5 mU/ml 20 mM Tris-HCl,
pH 7.2, 150 mM NaCl)
25 µl CaCl2 (25 mM)
25 µl rabbit brain cephaline (0.2 mg/ ml)
=> incubate for 1 min at 37 oC
25 µl human citrated plasma
Presentation
A five (5) vial set, each vial contains 5 U of purified Factor X activator from Russell's viper venom lyophilized in 20 mM Tris-HCl, 10 mM NaCl, 0.5% Prionex, pH 7.2. Reconstitute each vial with 1 ml filtered deionized water.
1 unit (U) is the amount of RVV-X which generates one international unit of enzyme (factor Xa) from zymogen (factor X) (2).
Storage and Stability
May be used by the expiry date given on the label when stored unopened, protected from moisture in the dark at 2°-8°C. Avoid contamination of the reagents by micro-organisms.
Stability after reconstitution:
![938915_ADG545](/uploads/artikeldetails/938916_adg545.jpg)
The reconstituted product can be frozen immediately and stored at -20°C. Avoid refreezing.
Category: Research use only
Type: Snake venom protease activator
Product Availability: Worldwide
Manufacturer: ImmBioMed GmbH & Co KG, Germany
For more information please click .pdf icon below.
RVV-X (Russell's Viper Venom, factor X activating protein)
Cat.No. ADG545
Article no.: 938916
Unit: 5 x 5 U
Code: ADG545
Manufacturer: ImmBioMed GmbH & Co. KG
References
- Application of snake venom proteins in the diagnosis of hemostatic disorders. In: Medical Use of Snake Venom Proteins, Stocker K. Stocker K, ed. Boca Raton: CRC-Press 1990; 213-252.
- Determination of factor X activator in the venom of the saw-scaled viper (Echis carinatus), Stocker K, Fischer H, Brogli M. Toxicon 1986; 24: 313-315.
- The use of dilute Russell’s viper venom time for the diagnosis of lupus anticoagulant. Thiagarajan P, Pengo V, Shapiro SS. Blood 1986; 68: 869-874.
- Coagulant protein of Russell’s viper venom. Furie B. Methods Enzymol 1976; 45: 191-205.