Fluorogenic Substrate PCa, fluorogenic substrate for activated Protein C

Description
Fluorogenic substrate for activated Protein C.

Chemistry
Formula: Pyroglu-Pro-Arg-AMC (C₂₆H₃₃N₇O₅ ∙C₂H₄O₂)
MW: 599.6 Dalton        Purity: >95% by HPLC
Absorption:  Maximum Wavelength 380_[ex] nm
Emission:     Maximum Wavelength 460_[em] nm

Principle

The initial rate of increase in the AMC concentration can be monitored fluorometrically at 380_[ex] nm and 460_[em] nm.

Assay conditions
Enzyme activity is determined by measuring the increase in absorbance of the free chromophore (AMC) generated, in comparison to the original substrate, per unit time 460_[em] nm. At excess substrate concentration, the rate of absorbance increases due to the amount of chromophore released is linearly related to enzyme concentration.
Measurement can be made either through acid quenching of the reaction (a. end-point method), or through use of a kinetic recording spectrophotometer (b. initial-rate-method).
 
Presentation
The vial contains 25 mg substrate, which has been lyophilized as an amorphous powder from acetic acid solution.
Due to the nature of the material, it may coagulate at the bottom of the vial to form resinous droplets during storage. However, this kind of appearance change is not related to any deterioration in the guaranteed quality.

Reconstitution
Can be reconstituted in methanol:water [1:9] up to a concentration of 25 mg/ml. Shake gently before use.

Storage
May be used by the expiry date given on the label when stored unopened, protected from moisture in the dark at 2-8 °C.
After reconstitution in DMSO, the solution is stable for more than one month if it is protected from moisture and kept in the dark at -20 °C.

Category: Research use only

Type: Substrate

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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