rabbit anti-human tissue factor IgG

Description
Tissue Factor (TF) is a 45 kDa transmembrane cell surface glycoprotein known for its role in initiating coagulation. TF is comprised of three domains: an extracellular domain (aa 1-219), followed by a hydrophilic spanning domain (aa 220-242) and a cytoplasmic tail (aa 243-263).
The antibody is derived from the sera of rabbits immunized with recombinant human Tissue Factor (product no. ADG45). The IgG fraction of immunserum was isolated by affinity chromato-graphy on Protein G-Sepharose.

Properties
The antibody binds to both native and recombinant human Tissue Factor.

Presentation
Screw-capped clear glass vial containing 1.0 mg of purified IgG lyophilized from 1 mL PBS with 200 mM mannitol, pH 7.5.

Reconstitution
Add 1 mL of filtered deionized water. Agitate gently for 5 minutes.

Storage and Stability
Store unopened vials at 2 - 8 °C until the expiration date shown on label.
Store reconstituted antibody in aliquots at -20 °C or colder. Stable for at least 6 months; avoid repeated freeze and thaw cycles.
 
Applications
ELISA
The antibody can be used as capture or detection antibody in ELISAs.
Western Blot Analysis
The antibody is suitable for Western blot analysis. A concentration of 0.1-0.5 μg/mL is recommended for the primary incubation.

Category: Research use only

Type: Antibody

Product Availability: Worldwide

Manufacturer: ImmBioMed GmbH & Co KG, Germany

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